The season opener was aired on September 18, 2008. Whereas the season ended on may 14, 2009.
| No. | Episode | Air date | ||
| 1 | Lazarus Rising | 9/18/2008 | ||
| 2 | Are You There God? It’s Me, Dean | 9/25/2008 | ||
| 3 | In the Beginning | 10/2/2008 | ||
| 4 | Metamorphosis | 10/9/2008 | ||
| 5 | Monster Movie | 10/16/2008 | ||
| 6 | Yellow Fever | 10/23/2008 | ||
| 7 | It’s the Great Pumpkin, Sam Winchester | 10/30/2008 | ||
| 8 | Wishful Thinking | 11/6/2008 | ||
| 9 | I Know What You Did Last Summer | 11/13/2008 | ||
| 10 | Heaven and Hell | 11/20/2008 | ||
| 11 | Family Remains | 1/15/2009 | ||
| 12 | Criss Angel Is A Douchebag | 1/22/2009 | ||
| 13 | Afterschool Special | 1/29/2009 | ||
| 14 | Sex and Violence | 2/5/2009 | ||
| 15 | Death Takes a Holiday | 3/12/2009 | ||
| 16 | On the Head of a Pin | 3/19/2009 | ||
| 17 | It’s a Terrible Life | 3/26/2009 | ||
| 18 | The Monster at the End of This Book | 4/2/2009 | ||
| 19 | Jump the Shark | 4/23/2009 | ||
| 20 | The Rapture | 4/30/2009 | ||
| 21 | When The Levee Break | 5/7/2009 | ||
| 22 | Lucifer Rising | 5/14/2009 |
